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Fig. 7. Changes in dPSR levels can alter the number of interommatidial cells late in pupal eye development. Drosophila eye discs were dissected and stained with an antibody to Discs large to visualize the membranes of the cone cells and primary pigment cells and the hexagonal lattice of secondary and tertiary pigment cells and bristle cells. Fifty hour APF eye discs from (A) yw control or (B,C) dPSR^FM1. dPSR^FM1 mutant discs show ommatidial fusions and disorganization, indicated by arrows. (D) dPSR^FM1 at 40 hour APF appear normal, indicating that the defects seen at 50 hours are likely to be due to cell loss. Apoptosis of interommatidial cells was visualized by TUNEL. (E,F) TUNEL-labeled nuclei (red) are seen at 49 hours APF in dPSR^FM1 eye discs (F), but not in yw discs (E). Lack of hid suppresses the dPSR^FM1 phenotype as observed in w; hid⁰⁵⁰¹⁴, dPSR^FM1/H99, dPSR^FM1 (G), and results in extra interommitidial cells, indicated by arrows, similar to w; hid⁰⁵⁰¹⁴/H99 eyes that lack hid alone (H). Overexpression of dPSR results in extra interommatidial cells. These are indicated with arrows in w; GMR-GAL4/UAS-dPSR (I).

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