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Figure 2


Fig. 2. Assembly of cxcr4b-expressing cells into the olfactory placode is perturbed in ody/ody embryos. Zebrafish ody/ody and ody/+ embryos were hybridized with cRNA probe against cxcr4b. (A,B,F,G) Dorsal views with anterior to the bottom. (C,D,H,I) Frontodorsal views with anteroventral to the bottom. (E,J) Frontal views with ventral to the bottom. At 12 and 14 hpf, the arrangement of cxcr4b-expressing cells in ody/ody embryos (F,G) is indistinguishable from that in ody/+ embryos (A,B). At 16 hpf, cxcr4b-expressing cells in ody/ody embryos disperse laterally (arrows in H) and anteroventrally (bracket in H) away from the lateral edge of developing neural tube. At 18 hpf, substantial numbers of cxcr4b-expressing cells are mispositioned ventrally (brackets in I). By 20 hpf, some cxcr4b-expressing cells (brackets in J) fail to join a cluster positioned at the correct site of the olfactory placode. Scale bar: 50 µm.





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