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Figure 4


Fig. 4. Quantification of olfactory neuron mispositioning. (A-L) Distances from the midline to the medialmost and lateralmost cells (medial and lateral limits), and distances from the ventral edge of the head to the ventralmost and dorsalmost cells (ventral and dorsal limits) in individual olfactory placodes were measured from the images of 24 hpf zebrafish embryos stained with anti-GFP antibody (e.g. Fig. 3). Percentages of olfactory placodes that display indicated medial (orange), lateral (green), ventral (red) and dorsal (blue) limits are shown as bar graphs in wild-type embryos (A,G; n=30 placodes), ody/+ embryos (B,H; n=22), ody/ody embryos (C,I; n=34), cxcr4b morphants (D,J; n=30), cxcl12a morphants (E,K; n=20) and cxcl12b morphants (F,L; n=18). Arrowheads indicate the averaged distance of each limit. *, P<0.05; **, P<0.01; ***, P<0.001, compared with wild-type; t-test.





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