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Figure 6


Fig. 6. Localized Cxcl12a misexpression displaces olfactory neurons ventrally. Confocal z-stacked images of zebrafish embryos labeled with anti-GFP (green) and anti-DsRed (magenta) antibodies. All panels show frontal views with dorsal to the top. (A) omp:yfp/+ embryo injected with phsp:mDsRed. Mosaic expression of mDsRed (magenta) does not affect positioning of YFP-expressing olfactory neurons (green). (B-D) Three examples of omp:yfp/+ embryos injected with phsp:cxcl12a-mDsRed. YFP-expressing olfactory neurons do not coalesce into a compact cluster. Some neurons (brackets and arrow) are found at positions close to the ventral ectopic sources of Cxcl12a-mDsRed (arrowheads). By contrast, no olfactory neurons are displaced to positions near the dorsal ectopic sources (open arrowheads). Scale bar: 100 µm.





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