|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. RGA-2 colocalises with actin microfilaments in the epidermis.
(A-C) Confocal lateral images of C. elegans embryos carrying
the rescuing rga-2::gfp fusion F1 (see
Fig. 3). (A) 1.2-fold, (B)
1.5-fold and (C) 3-fold stage embryos. GFP expression was restricted to the
epidermis; it initially highlighted cell-cell junctions (white arrows), plus
thin and short filaments that progressively became organised into a
circumferential filamentous network. (D) Confocal image of a small area
of the body from a rga-2::gfp transgenic 3-fold embryo showing GFP
fluorescence (green, left), bodipy-phallacidin staining to visualise actin
(red, middle) and merged picture (right) showing colocalisation. Arrows, actin
in underlying muscles; arrowheads, epidermal cell-cell junctions. (E-J)
DIC (top) and GFP fluorescence (bottom) pictures of adults, illustrating
expression in sensory support cells of the head (E,F), vulval cells (G,H) and
rectal cells (I,J), which are epithelial. Scale bars: 10 µm.
|
