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Figure 3


Fig. 3. Structural and functional requirement for actin colocalisation in C. elegans. (A) Schematic of the RGA-2 protein and deletion constructs analysed. All constructs were expressed under the control the rga-2 promoter. Black bars, constructs conferring strong colocalisation with actin. Right column, ability to rescue rga-2(hd102) lethality: +, rescue; -, no rescue; no sign, not tested. (B-G) Confocal lateral pictures of 3-fold embryos. (B) PH domain-containing F2 construct. Note the punctate localisation. (C) RhoGAP domain-containing constructs (construct P12 is shown). The distribution was mostly cytoplasmic, with regularly spaced and faint bands visible in areas where the epidermis is thinner (arrow). An enlargement of the boxed area is shown in C'. (D) Constructs lacking the final 46 residues (construct P6 is shown). The distribution is cytoplasmic. (E) Constructs containing the final 46 residues (construct P10 is shown). An enlargement of the boxed area is shown in E'. (F,G) P13 constructs with a CAAX or SAAX box, respectively, positioned after the GFP. The CAAX box confers disorganised membrane localisation; the SAAX box confers an apparently normal localisation; it is unclear why the presence of the SAAX box improves the faint actin localisation of construct P13 alone. Scale bar: 10 µm.





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