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Figure 6


Fig. 6. LET-502 and RGA-2 are dispensable beyond the 2-fold stage. (A) The percentage of lethality reflects larval and embryonic lethality. Temperature-shifts were performed at least three times as described in the Materials and methods. More than 60 individuals were sampled for each time point. Full genotypes: squares, let-502(sb118ts); circles, let-502(sb118ts) dpy-5(e61) rga-2(hd102). let-502(sb118ts) dpy-5(e61) behaved like let-502(sb118ts) but was omitted for clarity (data not shown). Black symbols, 25.5 to 20°C downshift; white symbols, 20 to 25.5°C upshift. LB, lima-bean stage (no elongation); C, comma/1.2-fold stage. (B) Genetic model for the position of rga-2 in the C. elegans embryonic elongation pathway, based on Tables 1 and 2 and on Fig. 5. (C) Developmental model for the role of RGA-2 in elongation (see Discussion).





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