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Fig. 1. Ac3 expression in the mouse olfactory system. (A) RT-PCR showing that Ac3 transcripts are present during development of the main olfactory and vomeronasal epithelia. (B) Western blot analysis of Ac3 expression in the main olfactory epithelium and olfactory bulb. The closed arrow indicates the position of the expected 200-210 kD Ac3 band. The open arrow indicates the Ac3 variant found in the olfactory bulb (155-170 kD). A weak non-specific band of 200 kD is present in wild-type and Ac3^-/- olfactory bulb extracts. (C) In situ hybridization of the olfactory epithelium shows Ac3 mRNA expression in OSNs of a P15 mouse. A control hybridization on a similar section from an Ac3-null mouse is shown in the insert. (D) Expression of Ac3 in the vomeronasal organ of a P0 mouse (insert shows the corresponding in situ hybridization of an Ac3-null animal). The signal was weaker than the one observed in the main olfactory epithelium. (E) Expression of Ac3 in the olfactory system of E12.5, 16 and 19 embryos. The two lower images represent antibody controls: ctr1, Ac3^-/- section; ctr2, pre-adsorbed antibody. (F-H) Immunohistochemistry (F, DAPI; G, OMP) on coronal sections shows that Ac3 (H) is highly enriched on OSN dendritic endings, but is also expressed on OSN axonal bundles and in glomeruli. Arrowheads in F show individual glomeruli. Scale bars: 60 µm. Cp, cribriform plate; Gl, glomerular layer; MOE, main olfactory epithelium; NL, nerve layer; OB, olfactory bulb; Sm, septum; VNE, vomeronasal epithelium.

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