DEV002691 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Expression of Nkx6.1 in the Ngn3 domain fails to restore insulin expression in Nkx6.1 mutant embryos. Immunofluorescence detection of insulin (red) (A-C). Nkx6.1^−/− embryos have reduced insulin cell numbers at E18.5 (B). Expression of the Pdx1-Nkx6.1 transgene in Nkx6.1^−/− embryos does not restore formation of insulin⁺ cells (C). Morphometric quantification of the insulin⁺ area over total pancreatic area at E18.5 reveals a similar reduction in insulin⁺ area in Nkx6.1^−/− embryos that carry or do not carry the Ngn3-Nkx6.1 transgene (n=4) (D). Scale bar: 50 μm

• Supplemental Figure S2 -

  Fig. S2. Pdx1-Nkx6.1 embryos display variant degrees of mosaic transgene expression. Immunofluorescence detection of Pdx1 (green) and β-galactosidase (red) in pancreata from two Pdx1-Nkx6.1 littermates at E18.5. Scale bar: 50 μm

• Supplemental Figure S3 -

  Fig. S3. Rescued beta-cells in Nkx6.1 mutant embryos express common beta-cell markers. Immunofluorescence detection of Pdx1 (A-D, red), Hb9 (E-H, red), Isl1 (I-L, red), IAPP (M-P, red), PC1/3 (Q-T, red) with insulin (green) in pancreas from wild-type, Nkx6.1^−/−, Nkx6.1^−/−;Pdx1-Nkx6.1 and Nkx6.1^−/−;Pdx1-Nkx6.2 embryos at E18.5. Embryos of all genotypes co-express these markers with insulin. Scale bar: 50 μm