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Fig. 7. Expression of Nkx6.1 or Nkx6.2 in the Pdx1 domain restores Myt1
expression in Nkx6.1 mutant embryos. (A-D)
Immunofluorescence detection of Myt1 (red) and insulin (Ins, blue) in
pancreata from wild-type (A), Nkx6.1-/- (B),
Nkx6.1-/-;Pdx1-Nkx6.1 (C) and
Nkx6.1-/-;Pdx1-Nkx6.2 (D) embryos at E14.5. (B-D)
Nkx6.1-/- embryos have reduced Myt1 expression (B), which
is restored by expression of the Pdx1-Nkx6.1 (C) or
Pdx1-Nkx6.2 (D) transgenes. (E) Morphometric quantification of
the number of Myt1+ cells over total pancreatic area in E14.5
embryos reveals an average reduction of Myt1+ cells in
Nkx6.1-/- to 14% of wild-type numbers (n=3). The
Pdx1-Nkx6.1 or Pdx1-Nkx6.2 transgene restores the number of
Myt1+ cells in Nkx6.1-/- mutants to 75% and 70%
of wild-type values, respectively (n=3). Values in E represent
averages ±s.e.m. Scale bar: 50 µm.
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