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Fig. 5. Gdnf expression is reduced in the
Npnt-null embryonic kidney at E11.5 but is normal at E10.5
and E13.5. (A-P) Transverse sections through
Npnt+/+ and Npnt-/- embryos. (A-H)
Expression at E11.5 of the genes indicated, as detected (A-F) by in situ
hybridization or (G,H) by immunostaining. (A,B) Note the apparent absence of
Gdnf RNA in the MM of the mutant (demarcated by dotted circles),
whereas the level of Gdnf expression appears comparable in the
adjacent limb bud (arrows) in mutant and wild-type embryos. (C-F) Note that
expression of the Eya1 and Six2 transcription factor genes
is similar in mutant and wild-type MM. (G,H) PAX2 protein is detected in both
the UB (solid arrowhead) and its branches (open arrowhead), as well as in the
MM. Note the lack of invasion of the UB into the MM of the Npnt
mutant (asterisk). (I-L) Expression at E10.5 and (M-P) at E13.5 of the genes
indicated, as detected by in situ hybridization. Note that Gdnf and
Eya1 expression appears comparable in Npnt+/+ and
Npnt-/- embryos at these stages, although the mutant
embryonic kidneys are smaller than normal at E13.5. Scale bars, 100 µm.
Abbreviations as in previous figures.
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