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Fig. 4. Mef2 controls slow fibre myofibril assembly cell-autonomously and
rescues the morphant. Detection of slow MyHC (red), Mef2 (blue) and FITC
or GFP (green) in manipulated 24-hpf zebrafish embryos. (A,B)
Transplantation of FITC-dextran-labelled cells (arrowheads, green) from
mef2d/c morphants into wild-type host (A) or from wild-type donor
into mef2d/c morphant host (B). Note the immature character of the
FITC-labelled transplanted fibres in A and the greater maturity of
transplanted cells in B, compared with their neighbours. (C)
Co-injection of hs-mef2d-IRES-GFP and mef2d/c MO followed by
heat shock at 22 hpf rescues myofibril structure in the GFP-expressing cells,
which also contain nuclear Mef2 (arrowheads). Asterisk indicates a
Mef2-positive skin cell. (D) Bar chart showing quantification of
transplantation results.
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