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Fig. 2. Postembryonic interaction of AMP1 and MP in
Arabidopsis. (A-D) Scanning electron micrographs showing
relative sizes of SAMs of indicated genotypes at 3 days after germination
(DAG). (E-L) Cleared whole-mount preparations viewed with DIC optics.
Expression of stem cell marker pCLV3::GUS in the SAM of the indicated
genotypes at 3 DAG (E-H) and 5 DAG (I-L). Note that expression of
pCLV3::GUS extends beyond the most-central domain in the
amp1 background. Numerical values of shoot meristem sizes are shown
in Table 1A. (M)
Discrete domains of pCLV3::GUS expression (arrowheads) in
amp1 are associated with the formation of multiple meristems.
(N) Formation of multiple SAMs (arrowheads) on expanded apices in
amp1 mutants, which are clearly not associated with leaf axils.
(O) Left to right: wild type, amp1-9, amp1-9 mpG92 and
mpG92. Note that amp1 mp double mutants are smaller than
amp1 mutants and have restored flower formation, which is defective
in mp mutants. (P,Q) Gene dosage-specific phenotypes of
amp1 mp double mutants. Note that the restoration of flower formation
is far more complete in a double mutant comprising a strong amp1 and
a weak mp allele (amp1-9 mpG92 in P) than in combination
with a strong mp allele (amp1-1 mpBS1354 in Q). (R-U)
Flower phenotypes. Normal flower organs in amp1 mutants
(amp1-9 in S), inflorescence with few, highly reduced flowers in
mpG92 mutants (U) and intermediate, fertile flowers in amp1-9
mpG92 mutants (T). (V-Y) Scanning electron micrographs of
inflorescences indicating that no size abnormalities were observed in
amp1 mutants. (Z-AC) Light-grown seedlings at 7 DAG. Note the
advanced stage of the lateral root system in amp1 mutants.
(AD-AG) Dark-grown seedlings at 14 DAG. Adventitious roots formed in
amp1-9 and amp1-9 mpG92 mutants (arrows point to
hypocotyl-root junction). Scale bars: 100 µm in A-D,V-Y; 50 µm in E-M; 1
mm in R-T; 5 mm in Z-AC; 2 mm in N,AD-AG.
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