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Figure 2


Fig. 2. The vascular network organizes itself independently of muscle. (A) Transverse sections of E9.5 forelimbs of Pax3GFP/+ mouse embryos were incubated with the PECAM antibody (red) to visualize vascular cells. GFP (green) reveals the Pax3-expressing cells. DAPI staining (blue) visualizes the nuclei. (B,C) E13.5 forelimbs from Pax3+/+ and Pax3-/- mutant mice were cut transversally and then incubated with the PECAM antibody. (D,E) E13.5 hindlimbs of Pax3+/+ and Pax3-/- mutant mice were cut transversally and hybridized with the Hif2{alpha} probe and then incubated with the MF20 antibody. The asterisks show ventral muscles in control limbs (B,D) and the equivalent sites corresponding to the absent muscles in Pax3-/- limbs (C,E). Both muscles and muscle-less sites are surrounded by the vasculature.





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