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Figure 3


Fig. 3. The location of endothelial cells delineates the future cleavage zones of muscle masses. (A-F) Transverse sections of wings from E5/HH26 (A,D), E5.5/HH27 (B,E) and E6/HH28 (C,F) chick embryos were hybridized with the Vegfr2 probe (blue) and then incubated with the MF20 antibody (brown). (D,E) High magnification of the future cleavage zone of the ventral masses from A,B. (F) High magnification of the cleavage zone of the ventral masses from C. (G-K) Wings from E5.5/HH27 chick embryos were cut transversely from proximal to distal areas along the forearm elements and then hybridized with the Hif2{alpha} probe (blue) followed by immunohistochemistry using the MF20 antibody (brown). The presence of Hif2{alpha}-positive cells at the future splitting site of the ventral muscle mass was estimated to extend over 300 µm in length (shown are example images running from proximal to distal). (L) At E6/HH28, the ventral muscle mass was well separated into the posterior and anterior masses. The arrow indicates the Hif2{alpha}-positive cells delineating a future cleavage zone in the anterior part of the central muscle mass - cleavage that will produce the central and proximal anterior masses.





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