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Fig. 7. PDGFB inhibits the expression of muscle markers. (A)
Transverse sections of wings from E5.5/HH27 chick embryos were hybridized with
the Pdgfb probe followed by immunohistochemistry with the MF20
antibody. (B,C) Transverse and consecutive sections of wings
from E5.5/HH27 chick embryos were hybridized with the Pdgfrß (B)
and MyoD probe (C). (D-I) PDGFB or PBS beads were implanted
into the dorsal regions of E5/HH26 wings and the embryos fixed 2 days or 2.5
days later, at E7/HH30 or E7.5/HH31. (D) Whole-mount preparations of
PDGFB-implanted wings hybridized with MyoD show inhibition of
MyoD expression around the bead. Sections of the PDGFB (E,I) or PBS
(F,G,H) -treated wings were hybridized with the MyoD (E-G) or the
Pdgfrß (H,I) probes. The expression of MyoD (E) and
Pdgfrß (I) was inhibited around PDGFB beads, whereas PBS beads
did not have any effect on MyoD (F,G) or Pdgfrß (H)
expression. The PBS bead (H) is shown at a more proximal level than the PDGFB
bead (I), explaining the difference of muscle pattern in ventral muscles
between limbs. The two anterior and ventral muscles visualized in the control
PBS limbs (H) are only observed in proximal region of the forearm. For all the
sections (A-C,E-I), top is dorsal and left is posterior; u, ulna; r,
radius.
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