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Figure 8


Fig. 8. PDGFB activates the expression of connective tissue markers. PDGFB or PBS beads were implanted into the dorsal regions of E5/HH26 chick wings and the embryos were fixed 2 days later, at E7/HH30. Consecutive sections of the PDGFB-treated wings (B,D) and of the corresponding control left wings (A,C) were hybridized with probes for the muscle connective tissue markers collagen I (A,B) and Tcf4 (C,D) and then incubated with the MF20 antibody. (E) Transverse section of an E6 wing showing the endogenous expression of Pdgfr{alpha} in muscle connective tissue. Pdgfr{alpha} transcripts (blue) are detected in the dorsal muscle mass (brown) in a future site of cleavage. (F) PDGFB-treated wings were hybridized with the Pdgfr{alpha} probe.





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