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Figure 9


Fig. 9. PDGFB increases cell density without modifying cell proliferation. Cell density around PBS (A) and PDGFB (B) beads were visualized with Hoechst-labeled nuclei at E7, 2 days after grafting into chick wings. (C,D) Adjacent sections to those shown in A,B, hybridized with the MyoD probe. There is an increase in cell density homogenously around PDGFB beads (B), where MyoD expression is inhibited (D), whereas PBS beads do not induce any cell accumulation (A) and do not modify muscle organization (C). It should be noted that cell density is higher in muscles compared with limb connective tissue at this stage (A-D). Transverse sections from PBS (E) or PDGFB (F,G) -treated wings incubated with the anti-BrdU antibody show that application of PDGFB does not modify cell proliferation around the bead. (G) The adjacent section to that shown in F, hybridized with the MyoD probe and then incubated with the MF20 antibody.





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