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Fig. 1. Reverse transcriptase-PCR analysis of CYLD expression in
Drosophila melanogaster. (A) Five transcripts of
Drosophila CYLD have been described, and are shown here. The
positions of the CYLD primers used in B (DCYF3 and DCYR6) are shown.
(B) CYLD (DmCYLD) expression was detected by reverse
transcriptase (RT)-PCR using the primers DMCYLDF3 (DCYF3) and DMCYLDR6
(DCYR6), and RNAs were isolated at the indicated developmental stages and in
the indicated body compartments of adult w1118 flies (upper panel).
RP49 (also known as RpL32) RNA was used as a control for the
amount of cDNA used in the RT-PCR reaction (lower panel).
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