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Fig. 7. Drosophila CYLD (DmCYLD) interacts with Kenny
(dIKK
). (A) Quantitative yeast two-hybrid assay with
all of the components of the IMD pathway (PGRP-LC, PGRP-LE, PGRP-LA, IMD,
dTAK1, dFADD, DREDD, IRD5 and Kenny). CYLD interacts specifically with Kenny,
the Drosophila ortholog of the mammalian protein NEMO (IKK
).
(B) Anti-V5 western blot of anti-c-Myc-immunoprecipitated material from
lysates of Drosophila S2 cells transfected with plasmids expressing
the proteins indicated on the top of the panel.