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Figure 1


Fig. 1. Disruption of the mouse Icap-1 gene. (A) Partial structure of the mouse Icap-1 gene and the targeted allele after homologous recombination. Black boxes represent exons (E2 to E7). The initiation codon (ATG) is located in exon 2. The expected fragment sizes for wildtype and recombinant alleles are 20 and 10 kb, respectively, following digestion with BamHI and hybridization with the indicated external probe (ext pb). (B) Southern blot analysis of tail DNA isolated from wild-type, heterozygous mutant and homozygous mutant mice. (C) Northern blot analysis of total RNA derived from adult kidney of wild-type, heterozygous and homozygous mutant mice. The filter was hybridized with probes specific for Icap-1 and Gapdh, respectively. (D) Western blot analysis of wild-type, heterozygous and homozygous mutant brain extracts. (E,F) Whole-mount lacZ staining of heterozygous mutant embryos at E8.5 (E) and E14.5 (F).





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