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Fig. 4. Defective formation of the osteogenic front in
Icap-1-/- calvaria. (A) Whole-mount
lacZ staining of newborn calvariae of Icap-1+/+
and Icap-1-/- mice. Strong ß-gal activity visualizes
Icap-1 expression in the sutural regions and the edges of the bony
plates of the Icap-1-deficient calvaria. (B,C) Hematoxylin and
Eosin-stained frontal section of the parietal region. The distance between the
ossified ends of the parietal bones (arrows) is wider in
Icap-1-/- (C) compared with wild-type mice (B).
(D-G) Frozen frontal sections through the sagittal suture of wild-type
(D,F) and Icap-1-/- (E,G) animals stained with Hematoxylin
and Eosin (D,E) or for ß-gal activity (F,G). Note that the wild-type
suture presents a typical condensed cell population corresponding to the
osteogenic front (arrowheads) that is severely reduced in the
Icap-1-/- mice. lacZ staining indicates extensive
expression of ICAP-1 in this region. B, bone; i.m, intersutural mesenchyme;
SO, supraoccipital bone; P, parietal bone; F, frontal bone; S, sagittal
suture; L, lambdoid suture; C, coronal suture; IF, interfrontal suture. Scale
bars: 2 mm in A; 50 µm in B-G.
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