DEV05611 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

  Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. stella expression in Blimp1-mEGFP-positive cells. Blimp1-mEGFP (green, left column), stella (red, middle column) and merged images with Hoechst (blue, right column) at the LS (E7.0; top row), EB (E7.25; second row) and EHF (E7.75; third row) stages. The percentage of stella-positive cells among Blimp1-mEGFP-positive cells at the respective stages are shown in the merged column. Scale bar: 50 μm.

• Supplemental Figure S2 -

  Fig. S2. Histone modifications in lineage-restricted PGC precursors. H3K9Ac (red, left column, top row), H3K4me2 (red, left column, second row), H3K4me3 (red, left column, third row), H3K9me1 (red, left column, fourth row), H3K9me2 (red, left column, fifth row) and H3K27me3 (red, left column, bottom row) in lineage-restricted PGC precursors (green, middle column) at E6.75. Images merged with DAPI staining (blue) are shown on the right column. Scale bar: 25 μm.

• Supplemental Figure S3 -

  Fig. S3. H3K9me1, H3K9me3 and H3K27me2 in PGCs at E8.5. H3K9me1 (red, left column, top row), H3K9me3 (red, left column, second row) and H3K27me2 (red, left column, bottom row) in PGCs (green, middle column) at E8.5. Merged images with Hoechst staining (blue) are shown on the right column. Scale bar: 10 μm.

• Supplemental Figure S4 -

  Fig. S4. Expression of JmjC domain-containing genes in PGCs and their somatic neighbors. Ct values, normalized by those for Gapdh, Ppia and Arbp, of the Q-PCR analysis of the expression of the genes examined in PGCs and their somatic neighbors at E7.25 and E7.75. PGCs at E7.25 are shown in pale green, those at E7.75 in green; somatic neighbors at E7.25 are shown in pale orange, those at E7.75 in orange.