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Figure 3


Fig. 3. Cell cycle distribution of migrating PGCs. (A) PGC number with average and standard deviation (top), ratio of PGCs with BrdU (middle) and with cyclin B1-strong-positivity (bottom) over the course of development in mice. After E8.25 (dotted line), randomly chosen cells were analyzed for BrdU incorporation and cyclin B1 immunostaining. (B) Representative images of BrdU incorporation (red) into PGCs (green) at E7.25, E7.75, E8.5 and E10.5 as indicated. (C) Representative images of cyclin B1 immunoreactivity (red, left column) in PGCs (green, right) at E7.75, E8.25, E9.25 and E9.75 as indicated. (D) FACS analysis of the cell cycle distribution of PGCs (green) and embryonic somatic cells (pink) during the course of development. Red arrowheads denote the G1 and G2 peaks. Scale bars: 10 µm in B,C.





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