|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. runx1 expression fails to initiate in the ALM and PLM of
zebrafish embryos compromised for Rad21, but late neuronal runx1
expression is Rad21-independent. (A-D) Whole-mount embryos stained
for runx1 expression. For all, upper panels are wild type, lower
panels are rad21ATGMO morphants (MO) or nz171 homozygotes as
indicated. (A) Posterior views of embryos, stages as indicated. rad21
morphants (MO; 0.5 pmol) at the 4-somite stage (4s; 14/15 embryos) and 6s
(23/25 embryos) were runx1 negative. Expression of runx1 in
RB cells is just detectable by the 8-somite stage in nz171 mutants, but PLM
expression remains absent throughout. (B) Lateral views, stages as indicated.
runx1 mRNA is not detected in the PLM or ICM, or in the olfactory
placode of nz171 mutants, but is present in a population of RB cells. (C)
Anterior views, stages as indicated. The occasional runx1-positive
cell is visible in the ALM of nz171 mutants. (D) Lateral views, stages as
indicated. At 24 h.p.f., nz171 homozygotes appear delayed and display no
runx1 expression. By 28 h.p.f., neuronal and olfactory placode
expression has initiated in mutants. Hematopoietic expression remains absent,
c.f. wild type, where expression of runx1 is observed in the ventral
wall of the dorsal aorta. Anterior is to the left for B and D.
|
