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Figure 4


Fig. 4. The C-cad G-A mutant does not rescue dense actin assembly when endogenous C-cadherin is depleted. (A) When overexpressed, C-cad G-A was localized on the cell surface, but the C-cad {Delta}CBD mutant was trapped in the intracellular vesicles in Xenopus embryos depleted of C-cadherin. (B) F-actin (green) and C-cadherin (red) double staining shows that the C-cad G-A mutant did not rescue the dense actin assembly. Arrows denote actin-rich membrane processes. (C) Overall levels of polymerized actin quantitated by pixel intensity. *, P<0.05. Scale bars: 50 µm in A; 20 µm in B.





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