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Figure 6


Fig. 6. Gain-of-function experiments show that the G-protein-coupled receptors Xflop and LPA2 control the level of C-cadherin on the cell surface. (A,B) Cadherin-stained animal caps from untreated animal caps (Control) and animal caps from Xenopus embryos injected with Xflop (A, right panel) or LPA2 (B, right panel) mRNA. (C) Quantitation of pixel intensity showing that both mRNAs increased the level of cadherin staining. *, P<0.05. (D) Western blot showing the increase in the total levels of C-cadherin caused by overexpression of Xflop and LPA mRNAs. {alpha}-tubulin was used as loading control. Scale bars: 20 µm.





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