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Figure 1


Fig. 1. Patterning defects in as mutants and 35S::BP Arabidopsis plants. (A,C,E) Stage 17 fruits of the wild-type Col accession. (A) Bright-field stereomicroscope image; (C) SEM; (E) cross section. (B,D,F) Stage 17 fruits of the as1-1 mutant, which produces bumpy fruits (B) and displays large repla both in SEM (D) and cross sections (F). (G,I,K) Bright-field stereomicroscope image (G) and cross sections (I,K) of stage 17 fruits of the Ler accession. (H,J,L) Bright-field stereomicroscope image (H) and cross sections (J,L) of stage 17 fruits of as1-104. Notice the increased size of repla and the reduced size of valves in as1-104 fruits (I-L). (M,N) Cross sections of stage 17 fruits of as2-1 (M) and the 35S::BP line (N), showing the large replum phenotype also seen in as1-1 (D,F). (O,P) Cross sections of stage 17 fruits of the as1-1 bp-1 (O) and as1-104 bp-1 (P) double mutants that show strong recovery of the wild-type replum phenotype. (Q,R) Lignification pattern of stage 18 fruits of Col (Q) and as1-1 (R) stained with phloroglucinol. (S-U) GUS expression in the valve margin driven by the SHP2 promoter in Col (S,T) and as1-1 (S,U) fruits at stage 15. Fruits in the pictures are in the ER background, with the exception of those in G-L and P, which are in the Ler background. Arrowheads indicate the positions of valve margins. Scale bars: 2 mm in A,B,S; 1 mm in G,H; 500 µm in Q,R; 200 µm in I,J; 100 µm in C-F,K-P,T,U. r, replum; v, valve.





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