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Fig. 7. Retinoic acid treatment counteracts the developmental mdDA defects in
Pitx3 deficiency. (A) Schematic representation of an E14.5
sagittal section showing the position of the caudal (I) and rostral (II)
sections shown in B and C. The position of the meso-diencephalic dopaminergic
(mdDA) neurons are depicted in gray. (B) E14.5 coronal sections of the
caudal (A-C) and rostral (D-F)
part of the mdDA area. (B,E) Ahd2 protein distribution
(red). MdDA neurons were identified by the expression of TH protein (green;
A,D). Overlays demonstrate the co-expression of the TH
and Ahd2 proteins in a specific subpopulation of mdDA neurons located in
rostro-lateral positions and defining the developing substantia nigra pars
compacta (SNc; C,F). (C) Distribution of
TH-immunoreactive (TH-IR) neurons in E14.5 coronal brain sections of retinoic
acid (RA)-treated Pitx3-/- embryos (B,F),
RA-treated Pitx3+/+ embryos (D,H),
untreated Pitx3-/- embryos (A,E) and
untreated Pitx3+/+ embryos (C,G) at
the caudal (A-D) and rostral (E-H)
level of the mdDA area. (D) Quantitative analysis of TH-IR neurons in
the caudal and rostral part of the mdDA area in RA-treated (black bars) and
untreated (white bars) embryos. The average number of TH-IR neurons per
section are expressed as a percentage of the number of
Pitx3+/+ embryos ±s.e.m. (n=3; Student's
t-test; *P<0.01). F, forebrain; H, hindbrain;
M, midbrain; MHB, mid/hindbrain border.
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