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Fig. 5. Altering the SYS-1-to-POP-1 ratio also affects cell fate decisions at
other A-P divisions. (A) Schematic of the C. elegans
pharynx, including the location of the eight pharyngeal muscle types (labeled
1 through 8), with 3NB12 staining indicated (dark gray, no staining).
(B) Embryos derived from wild type,
teEx321(Pmed-1gfp::sys-1) and sys-1(q544/+);
teIs3(Pmed-1gfp::pop-1) stained with 3NB12. Schematics to the
right indicate the three pm6 and three pm7 muscle cells in the wild type and
predicted cell fate changes following an anterior-to-posterior (middle) or a
posterior-to-anterior (bottom) fate transformation at the division giving rise
to MSa and MSp. Triangles and squares denote cells derived from the MSa and
MSp lineages, respectively. (C) The E lineage. Horizontal lines
indicate divisions, whereas vertical lines indicate developmental time. All
divisions are anterior (left) to posterior (right), except those labeled l/r,
which indicate left/right divisions. Circles, the four cells that comprise
intestinal ring 1; triangles, the two cells comprising ring 9. (D)
PHO-1::GFP expression in wild-type and teEx321 larvae. Arrow,
posterior end of the pharynx; brackets, cells in the first two intestinal
rings in wild type and the corresponding region in teEx321. Scale
bar: 10 µm in B; 30 µm in D.
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