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Figure 1


Fig. 1. Generation of an Scx null allele. (A) Schematic representation of alleles generated in the Scx locus. Black boxes represent coding regions of the Scx gene, and open boxes represent the 5' and 3' untranslated regions (UTRs). The recognition sequences for the Cre and FLP recombinases, the loxP (green boxes) and FRT (orange boxes) sites, respectively, are shown. The brown box represents the neomycin-resistance (Neo) cassette used for negative selection during targeting in embryonic stem cells (see Materials and methods for details). (B) Representation of the unique organization of the Scx locus. The two exons of the Scx gene (A) are located within the third intron of a second gene called block of proliferation 1 (Bop1), which is transcribed in the opposite orientation. (C) Scx in situ hybridization on cross sections through the digits of wild-type (WT) and Scx-/- embryos at E15.5.





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