|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. The long force-transmitting tendons and intermuscular tendons are
severely disrupted in Scx-/- mice. Tendons in
wild-type and mutant littermates were visualized directly in skinned tissues
using the ScxGFP transgenic reporter (C-L). (A) The forelimbs
of an Scx-/- newborn mouse at postnatal day (P)3 are
locked in a dorsal flexure. (B) Forelimbs of P14 wild-type (WT) and
Scx-/- mutant mice stained with Alcian blue and alizarin
red. Black arrowheads point to the deltoid tuberosity, which is missing in the
mutant. (C,D) The dorsal extensor tendons of the forelimb.
Yellow arrowheads show the extensor digitorium communis (EDC). Long and short
purple arrowheads show the extensor digiti quinti and extensor carpi ulnaris,
respectively. (E,F) The ventral flexor tendons of the forelimb
in wild type (E) and mutants (F). (G,H) Tail tendons at P14
visualized by direct illumination (G) or by fluorescence of the
ScxGFP reporter (H). Yellow arrowheads show the tail tendons. Pink
arrowheads show the annulus fibrosus. (I,J,M,N)
Tendons of the trunk at E18.5. (I,J) Dorsal view of skinned trunks. (M,N)
Sagittal trunk sections stained with an antibody to myosin heavy chain (MHC).
Pink arrowheads show short-range tendons. Yellow arrowheads show long tendons.
(K,L) The intermuscular tendons of the subscapularis muscle at
E18.5 seen in a ventral view of the scapula. (O-T) Frontal sections
through the trunk of E18.5 wild-type and Scx-/- embryos at
the level of the diaphragm, carrying the ScxGFP reporter and
counterstained with an MHC antibody. Yellow arrowheads show the middle tendon
of the diaphragm. (O,P,S,T) Comparison of the signal of ScxGFP (O,P) and in
situ hybridization with a collagen I probe (S,T) in the diaphragm tendon from
a wild-type and a mutant embryo. (Q,R) Expression of tenascin C and collagen
XII in the diaphragm tendon of a mutant embryo.
|
