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Fig. 1. Postnatal pancreatomegaly after Apc loss. (A) An E10.5 double-transgenic R26R;Pdx1-Cre^+/- embryo stained with X-gal (whole mount). ß-galactosidase activity is detected both in the ventral and dorsal pancreatic buds. (B) Genomic PCR using DNA from tail biopsies, isolated acini, ducts or islets of 2-month-old control (+/+ and +/-) and Apc^P-/- mice. Apc is efficiently recombined in all pancreatic cell types in Apc^P-/- adults (300 bp band; unrecombined tissues yield a 1 kb-long PCR fragment). (C) Freshly dissected pancreas of 2-month-old Apc^loxP/loxP (control) and Apc^P-/- mice. (D) Pancreas:body-weight ratio. The pancreatic mass is increased in Apc^P-/- mice (pancreatic weight: control, 0.39±0.108 g; Apc^P-/-, 1.19±0.124 g). In total, six mice were analyzed per group; ^***P<0.001. (E) Cell proliferation rate. The proliferation index was assayed with anti-BrdU or anti-Ki67 antibody, as indicated. White bars, controls; black bars, Apc^P-/- mice (n=3 individuals per group; ^**P<0.01; ^*P<0.05). vp/dp, ventral/dorsal pancreatic primordia; s, stomach; d, duodenum; p, pancreas; sp, spleen. Scale bar: 0.5 cm in C.

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