|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Stat6 is upregulated and activated at the onset of pregnancy.
(A) Protein was extracted from inguinal mammary glands from virgin
mice; from mice at day 5, 10 and 15 of gestation; and from mice at day 0 (day
of birth), 5 and 10 of lactation. Immunoblotting was performed for
phosphorylated Stat6 (pStat6), Stat6, IL-4R
, Gata3, c-Maf and
-tubulin. pStat6 and Stat6 immunoblots were quantified using ImageJ
software and plotted on a histogram (right). (B-I) Immunohistochemistry
(IHC, right panels; left panels, merged with DAPI staining) was performed on
mammary gland sections from wild-type mice for: pStat6 in virgin mice (B) and
mice at day 5 of gestation (C); IL-4R in virgin mice (D) and mice at
day 15 of gestation (E); Gata3 in mice at day 5 of gestation (F); and c-Maf in
mice at day 15 of gestation (G). (H) pStat6 staining in
Stat6-/- mice at day 5 of gestation. (I) Rabbit isotype
control staining on wild-type mice at day 5 of gestation.
|
