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Figure 3


Fig. 3. Reduction in proliferation and in the levels of differentiation markers in Stat6-/- mice. (A) Immunoblots for Stat6, phosphorylated (p)Akt, Akt, pERK1/2, ERK1/2, ß-casein, pStat5, Stat5, Gata3, Pten, IL-4R{alpha} and {alpha}-tubulin were performed on wild-type (WT) and Stat6-/- tissue extracts at the indicated time points. (B) Immunoblots from A were quantified using ImageJ software and plotted on a histogram. (C) A microarray analysis of differentially regulated genes at day 5 of gestation comparing wild-type and Stat6-/- mammary glands. Genes listed displayed a statistically significant change across three biological replicates with a P value of <0.01.





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