spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. IL-4/IL-13 expression is reduced in Stat6-/- mammary glands and these cytokines are required for mammary gland development. (A) Quantitative real-time-PCR was performed using specific primers for Il4, Il13, Il5, Il12a and Gata3 on wild-type (WT) and Stat6-/- glands at day 5 and 15 of gestation. Error bar represents s.d. of three samples and is representative of at least two experiments. (B-D) Whole mounts (left panels) and H&E-stained sections (right panels) of inguinal mammary glands from wild-type (strain matched) and IL-4-/-/IL-13-/- mice at day 5 (B), 10 (C) and 15 (D) of gestation. (E) The number of alveoli was scored in H&E images from wild-type and IL-4-/-/IL-13-/- mice at 40x magnification and the area of the fat pad was measured using ImageJ software. The number of alveoli/mm2 was calculated and the percentage relative to wild-type was plotted. (F) Immunoblots for phosphorylated (p)Stat6 and Stat6 were performed on wild-type and IL-4-/-/13-/- mice at day 5 of gestation. The density of the bands was quantified using ImageJ software and the ratio of pStat6 to Stat6 plotted on a histogram.





Right arrow Return to article