DEV00141 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Expression of Foxa1/2 in the ventral midbrain. Co-expression of Foxa1/2 in all ventral midbrain progenitors except for a few cells near the alar/basal plate boundary (arrows). Foxa2 is also expressed in Brn3a⁺ red nucleus, but not in Islet1⁺ oculomotor neurons. Scale bars: 75 μm.

• Supplemental Figure S2 -

  Fig. S2. Cell proliferation and cell death are not affected in Foxa1^−/−;Foxa2cko double-mutant embryos at E12.5. Similar numbers of BrdU⁺ (A,B) and TUNEL⁺ (C,D) cells in control and Foxa1^−/−;Foxa2cko double-mutant embryos. Scale bars: 75 μm.

• Supplemental Figure S3 -

  Fig. S3. Development of other neuronal subtypes in the ventral midbrain at E12.5. Reduced numbers of Islet1⁺ OCM (top) and Brn3a⁺ red nuclei neurons (middle), in contrast to normal numbers of GABAergic⁺ neurons (bottom) in the ventral midbrain of Foxa1^−/−;Foxa2cko double mutants as compared with control embryos.