spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. E-C-coupling components are dramatically decreased in ryr fast muscles. (A-H,K-R) The distribution of RyRs and DHPRs were assayed in fast and slow muscles at 48 hpf with anti-RyR (A,B,K,L), anti-DHPR{alpha}1 (C,D,M,N), anti-DHPRß (E,F,O,P) and anti-DHPR{alpha}2 (G,H,Q,R). Wild-type fast muscles express RyR (A), DHPR{alpha}1 (C), DHPRß (E) and DHPR{alpha}2 (G) in a striated pattern that presumably corresponds to the t-tubule-SR junctions, whereas clustering is not observed in mutant fast muscles (B,D,F,H). Wild-type slow muscles express RyR (K), DHPR{alpha}1 (M), DHPRß (O) and DHPR{alpha}2 (Q) in a striated pattern, as in wild-type fast muscles. ryr mutant slow muscles also express RyR (L), DHPR{alpha}1 (N), DHPRß (P) and DHPR{alpha}2 (R) in a striated pattern. (I,J,S,T) RyR (green) and DHPR{alpha}2 (purple) are colocalized in wild-type fast (I) and slow (S) muscle and in mutant slow muscle (T) but not in ryr mutant fast muscle (J). Insets show a higher magnification of muscle fibers. (U-X) Electron micrographs of t-tubule-SR junctions in muscles at 48 hpf. Putative RyR-DHPR aggregates are visible as dense particles between t-tubule and SR membranes in wild-type fast muscle (U) but not in ryr mutant fast muscle (V). The RyR-DHPR aggregates are present in both wild-type (W) and mutant (X) slow muscles. SR, sarcoplasmic reticulum.





Right arrow Return to article