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Fig. 6. Mouse-turtle interspecies grafting experiments reveal conserved guidance
mechanisms in mammals and reptiles. (A-B') When DiI-labeled
explants of PSB and MGE of stage 16 to 18 turtle embryos were grafted into
E13.5 mouse forebrain slices, the released labeled cells (red) migrated within
the host tissue. Cells from PSB explants grafted into the corticostriatal
boundary (A) migrated along the corticostriatal boundary and accumulated
ventrally (arrow), whereas MGE cells grafted into the basal telencephalon
(B,B') migrated in the orthogonal direction, across the corticostriatal
boundary. Turtle MGE cells dispersed and migrated as individuals to colonize
the mouse cortex (B') and some developed branched neurites in the host
tissue (B''). (C-F) GFP-expressing MGE explants from E12.5 mouse
embryos grafted into stage 16 turtle slices (C) or into stage 17 telencephalic
vesicles in ovo (D) were no longer visible after a few days. Individual
GFP-positive mouse MGE cells migrated long distances within the telencephalon
of turtle embryos, colonized the entire slice (C), or the pallium in the in
ovo experiments (D). By contrast, cortical explants (E,F) still formed a
compact mass of tissue several days after grafting and released very few
cells. The few cells that were released did not migrate very far, either in
grafted slice (E) or grafted hemisphere (F). Scale bars: 500 µm in A,B,C-F;
200 µm in B'; 40 µm in B''.
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