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Fig. 4. Reduction of FLC expression in bri1 ld, bri1
fca and bri1 FRI double mutants efficiently accelerates
flowering. (A) The effect of vernalization treatment on flowering
time in the following mutants: bri1-201, ld-3, fca-11, FRI in the Ws
background, bri1-201 ld-3, bri1-201 fca-11 and bri1-201 FRI.
Germinated seedlings were vernalized for 6 weeks under short days (8 hours
light/16 hours darkness) at 4°C, after which they were grown under the
same long-day conditions as control non-vernalized plants. White bars,
non-vernalized controls; black bars, vernalization treatments. Absolute
(B) and relative (C) FLC mRNA levels before (-; white
bars) and after (+; black bars) vernalization. (D) Flowering phenotypes
of four independent FLC-RNAi transgenic lines in the bri1-201
ld-3 double-mutant background, compared to two independent lines of
bri1-201 ld-3 double mutants transformed with a control vector.
7-week-old plants are shown. (E) Flowering-time analysis of
FLC-RNAi bri1 ld mutants grown under a long-day photoperiod.
A representative result is shown. (F) RNA-blot analysis of FLC
mRNA levels in 20-day-old FLC-RNAi bri1 ld mutants compared
to the control bri1 ld lines. The ACT1 gene was used as a
loading control.
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