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Fig. 6. Histone acetylation at the FLC locus is increased in bri1
ld mutants. (A) Regions of the FLC locus examined by
ChIP. Coding regions are indicated with vertical lines, and the nine fragments
amplified with PCR are depicted and numbered from I to IX. (B) ChIP
with antibodies against trimethylated histone 3 at lysine 4 (triMeH3K4).
21-day-old Ws, bri1-201, ld-3 and bri1-201 ld-3 mutants
grown under long days were analyzed, and region IV was examined. (C)
ChIP with antibodies against acetylated histone 3 (H3Ac). Samples are as
described in B. (B,C) Input, denotes DNA amplified from each chromatin sample
before immunoprecipitation. -, denotes samples immunoprecipitated with
antibodies against anti-rat-IgG (negative control). Anti-H3Ac refers to
fragments amplified from chromatin samples immunoprecipitated with antibodies
against H3Ac, whereas Anti-triMeH3K4 refers to samples immunoprecipitated with
antibodies against trimethylated H3K4. UBQ10 served as an internal
ChIP control. Representative ChIPs are illustrated. Fold enrichment calculated
for a biological replicate is the mean of averaged enrichment ratios from two
independent immunoprecipitations. Fold enrichment in Ws was arbitrarily set as
1.
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