spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. ofs mutants fail to undergo meiosis or differentiation. (A-C) Phase images, apical-tip oriented to the left. (A) Heterozygous testes appear wild-type, with elongating spermatids filling most of the testis. (B) Mutant testes lack elongating spermatids. (C) ofs/Df(3R)mbc-R1 is indistinguishable from ofs/ofs (B). (D,E) High-magnification, with diagrams of the represented stages. Color code: primary spermatocyte nucleus, red; nucleolus, black; haploid spermatid nucleus, orange; fused mitochondrial derivative, purple; dissociated mitochondria, blue; cell outlines (where discernable), gray. (D) Heterozygous testes have examples of mature primary spermatocytes (left) and onion-stage spermatids (right). (E) The most prevalent cells found in ofs testes resemble primary spermatocytes. (F,G) Whole-mount immunofluorescent images of wild-type (F, arrowhead, 2xlacZ+) and ofs (G, arrow, lacZ-) clones in a heterozygous background. Flies were aged 4 days after clone induction, which allowed clones to progress midway through the meiotic G2 of wild-type spermatocytes. (F',G') Brackets are the same size in each panel, highlighting the cell size difference between the two clones. The entire ofs cell outline (PTyr, phospho-Tyrosine) is approximately the same size as the nucleus of the control cell.





Right arrow Return to article