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Figure 2


Fig. 2. Evc localises to the basal body and is not required for ciliogenesis. (A) Acetylated tubulin and {gamma}-tubulin immunofluorescence (green) distinguish the ciliary axoneme and the two centrioles, respectively, in mIMCD3 cells. Anti-Evc (red) reveals Evc to be localised at the base of the cilium. Arrowheads, centrioles. (B) {gamma}-tubulin (green) and Evc (red) immunofluorescence show that Evc signal overlaps with one of the two centrioles. (C) In situ Evc immunostaining of wild-type and Evc-/- chondrocytes of P0 proximal tibia demonstrate Evc localisation, the presence of cilia in the mutant and the specificity of the antibody. Scale bar: 2 µm.





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