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Fig. 2. Loss of Egfl7 results in delay of vascularization.
(A-D) CD31 wholemount staining on embryonic mouse hearts from
Egfl7+/+ (A,B) and Egfl7-/- (C,D)
littermates at E12.5 (A,C, dorsal view of the left ventricles) and E14.5 (B,D,
lateral view of the left ventricles, dorsal is to the right). Asterisks, areas
without coronary vasculature; weak signal is from the underlying endocardium.
(E,F) CD31 wholemount staining on embryonic heads from
Egfl7+/+ (E) and Egfl7-/- (F)
littermates at E10.5. Open arrowheads, major cranial vessels. (G)
Ventral coronary vascular coverage of multiple pairs of
Egfl7+/+ and Egfl7-/- littermates at
E12.5 and E14.5. Horizontal bars represent means. Because of large
inter-litter variation at E12.5, littermate relationship is specified with
numbers in the graph. Significant difference is marked with an asterisk next
to each P value. (H) Major cranial vessel counts of multiple
pairs of Egfl7+/+ and Egfl7-/- E10.5
littermates from two knockout lines. ins, insertional knockout line; hom,
homologous recombination knockout line. (I-N) Isolectin B4 (EC marker)
staining on flatmount neonatal retinas from Egfl7+/+ (I-K)
and Egfl7-/- (L-N) littermates at P2 (I,L), P5 (J,M) and
P8 (K,N). (O,P) Retinal vascular coverage (O) and size (P) of
multiple pairs of Egfl7+/+ and
Egfl7-/- newborn littermates at the indicated ages. Solid
red arrowheads indicate example aberrant EC clusters; arrows indicate sprouts
at the retinal vascular migration front. The actual size represented by the
width of the panel: 0.9 mm (A,C); 1.4 mm (B,D); 3.6 mm (E,F); 3 mm (I,J,L,M);
4.7 mm (K,N); K and N are assembled from overlapping 4x images.
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