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Fig. 5. EGFL7 restricts the spatial distribution of migrating ECs. (A-F) Confocal images of vascular sprouts in P5 retinas from Egfl7^+/+ (A-C) and Egfl7^-/- (D-F) littermates stained with Isolectin B4 (white in A,D; green in B,C,E,F) and propidium iodide (PI) to indicate nuclei (red in B,C,E-F). C and F are z-sections of the retinal vascular migration front. These images were taken from areas similar to those indicated by the arrows and arrowheads in Fig. 2J,M. Crosses mark stalk cell nuclei; asterisks mark tip cell nuclei. (G,H) Tip (G) and stalk (H) cell counts in multiple 40 µm-long segments of sprouts from both genotypes. (I-L) Aortic rings isolated from Egfl7^+/+ (I,J) and Egfl7^-/- (K,L) littermates stained for CD31 (green) and with DAPI (red, pseudo color). White dashed lines outline the edges of the aortic rings. The CD31^- DAPI⁺ signals in J and L are nuclei of fibroblasts and smooth muscle cells that have migrated away from the aortic rings. (M) EC counts in multiple 50 µm-long segments of sprouts from both genotypes. The actual size represented by the width of the panel: 230 µm (A,D); 76 µm (B,E); 298 µm (C,F); 1.3 mm (I,K); 294 µm (J-L).

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