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Fig. 6. Aberrant localization of basement membrane marker in the Egfl7^-/- vessels. Neonatal mouse retinas (A-F) or aortic rings (G-L) isolated from the Egfl7^+/+ (A-C,G-I) and Egfl7^-/- (D-F,J-L) littermates stained for collagen IV (green), CD31 (red) and with DAPI (blue). Representative single-frame confocal images (A,B,D,E,G,H,J,K) and optical z-sections (C,F,I,L) are shown. Arrowheads, vascular sprouts similar to those shown at higher magnification in the panels beneath; arrows, mislocalized collagen IV between ECs within a single sprout; asterisks, individual ECs. In C and F, two separate z-sections are shown in each panel. The actual size represented by the width of the panel: 732 µm (A,D); 108 µm (B,E); 36 µm (C,F); 366 µm (G,J); 72 µm (H,I,K,L).

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