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Figure 1


Fig. 1. Inactivation of TAF4 in foetal epidermis. (A,B) Gross morphology and X-Gal dye diffusion assay of E18.5 mice of the indicated genotypes. (C) Immunostaining of skin biopsies of E18.5 animals of the indicated phenotypes with anti-TAF4 antibody. The epidermis (E), dermis (D) and basal keratinocyte layer (BK) are indicated. Arrows indicate representative nuclei. Left panel, immunostaining; right panel, Hoechst stain. (D) Histological analysis of representative Haematoxylin-Eosin-stained paraffin sections from dorsal or ventral (upper and lower panels, respectively) regions of control and mutant foetuses at E18.5. The epidermis is indicated and the arrows indicate the location of the basal membrane. (E) Transepidermal water loss (TEWL) of three independent E18.5 foetuses of each genotype after 20 or 60 minutes as indicated. (F) Weight loss of three independent E18.5 foetuses of each genotype after the indicated times. (G) Quantitative RT-PCR on RNA from three independent E18.5 foetuses of each genotype. Duplicate reactions were performed for each RNA preparation. In each case, the expression in the Taf4lox/+ animals is set as 1. Scale bars: 30 µm in C; 50 µm in D.





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