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Figure 4


Fig. 4. Changes in gene expression upon TAF4 inactivation. (A,B) A restricted list of up- (A) or down- (B) regulated genes are shown along with their log2 values classified by their function or intracellular location. (C) RT-PCR analysis of gene expression of the indicated genes in RNAs from skin biopsies from two independent oil- or Tam-injected mice. (D) RT-PCR analysis of gene expression of the indicated genes in RNAs from skin biopsies of two independent E18.5 foetuses. (E) Immunoblot analysis of CDKN1A and cyclin D1 expression in epidermal extracts. 20 µg of total extract was loaded in each lane and the presence of CDKN1A, cyclin D1 and p53 revealed using the appropriate antibodies. In the lower part of the panel, phosphorylated p38 and CREB/ATF1 were revealed in total epidermal extracts prepared and quantified as described in Materials and methods.





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