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Fig. 1. Phenotype of LONESOME HIGHWAY mutants. (A)
Cross-section diagram of a mature Arabidopsis root; tissues are
arranged radially from outside in: epidermis (white), cortex (purple),
endodermis (dark blue) and stele [which consists of a ring of pericycle cells
(light blue) that surrounds xylem (yellow) and phloem (red) arranged in
bilateral symmetry]. (B,C) Confocal images of wild type (WT, B)
and lhw-1 (C) expressing the xylem-associated pericycle marker
J0121::GFP (green). Roots are counterstained with propidium iodide (PI, red)
to visualize the outlines of cells. (D) Bright-field image of a
lhw-1 root, which has all lateral roots (black arrowheads) emerging
from a single side of the primary root. (E,F) Confocal image of
basic fuchsin staining of xylem in wild type (E) and lhw-1 (F).
(G,H) Confocal images of wild type (G) and lhw-1 (H)
expressing the phloem marker APLpro::APL-GFP (green). (I,J)
Whole-plant phenotypes of wild-type Col (I) and lhw SALK_079402 (J).
(K) Root growth of wild type (left) and lhw-1 (right) on agar
plates at 20 dpg. Notice the root-waving and short-root phenotypes in
lhw-1. (L,M) Vascular pattern in mature (13 dpg)
wild-type (L) and lhw-1 (M) cotyledons. (N,O)
Higher-magnification images of xylem from images L and M, respectively. The
black arrow points to the end of mature xylem elements; to the right,
elongated cells typical of procambium are still seen. For each marker, the
wild-type and lhw image pair are at the same magnification.
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