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Fig. 2. Cross sections of lhw roots and hypocotyls. (A)
Schematic of stele in cross sections from wild type (WT), lhw and
wol-1; pericycle is in blue and xylem in yellow; wild type and
lhw were traced from 2F and 2G, respectively. (B-G)
Bright-field images of wild-type and lhw root sections taken at
increasing distance from the tip of the root; for each, the same pericycle
cell is marked with a blue star for orientation: (B) wild type, 30 µm; (C)
lhw-2, 30 µm; (D) wild type, 60 µm; (E) lhw-2, 60
µm; (F) wild type, 120 µm; (G) lhw-2, 120 µm.
(H,I) Toluidine-blue staining of wild type (H) and
lhw-2 (I) in the mature zone. (J,K) Section through the
lower third of the hypocotyl in wild type (J) and lhw-2 (K).
(L) Center of a wol-1 root, 66 µm from the tip. (M)
Center of a wol-1;lhw-1 root, 66 µm from the tip.
(N,O) DIC (N) and toluidine-blue (O) images of
wol-1;lhw-1 mature roots, showing the very reduced stele filled with
xylem elements. Each image pair is at the same magnification. Scale bars: 20
µm.
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